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Synthetic Biology: Tools to Design, Build, and Optimize Cellular Processes (pdf) [YKG]

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 							The general central dogma frames the emergent properties of life, which make biology both necessary and
difficult to engineer. In a process engineering paradigm, each biological process stream and process unit is heavily
influenced by regulatory interactions and interactions with the surrounding environment. Synthetic biology is developing
the tools and methods that will increase control over these interactions, eventually resulting in an integrative
synthetic biology that will allow ground-up cellular optimization. In this review, we attempt to contextualize the areas
of synthetic biology into three tiers: (1) the process units and associated streams of the central dogma, (2) the
intrinsic regulatory mechanisms, and (3) the extrinsic physical and chemical environment. Efforts at each of these three
tiers attempt to control cellular systems and take advantage of emerging tools and approaches. Ultimately, it will be
possible to integrate these approaches and realize the vision of integrative synthetic biology wh